Journal:

Article Title: Distribution of Aldoxime Dehydratase in Microorganisms

doi:

Figure Lengend Snippet: Aldoxime dehydration and nitrile-hydrolyzing activities in nitrile-degrading microorganisms when they were grown with or without inducers a

Article Snippet: No aldoxime dehydratase activity was seen when the strains were grown without aldoximes or nitriles, although PAN- or CyPy-hydrolyzing activity was detected in the cells. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Strain a Enzyme activity (U/liter of culture) b Source or reference PAOx dehydration PAN hydrolysis PyOx dehydration CyPy hydrolysis PAOx PAN None PAOx PAN None PyOx CyPy None PyOx CyPy None Alcaligenes faecalis IFO 13111 (ATCC 8750) – – – – – – 0.76 0.39 – 0.41 * 0.65 * 0.29 * 27 Brevibacterium butanicum ATCC 21196 – – – – – – 0.45 0.20 – 1.30 * 0.08 * 0.09 * 27 Corynebacterium alkanum ATCC 21194 0.23 0.08 – 4.21 * 0.83 * 0.67 * 0.51 0.21 – 1.33 * 0.66 * 0.38 * 27 Corynebacterium nitrilophilus NCIMB 11594 – – – – – – 0.21 0.13 – 0.35 1.10 0.06 18 Corynebacterium sp. strain C5 TPU 6015 3.32 6.08 – 10.7 * 27.2 * 8.39 * – – – – – – 22 Pseudomonas fluorescens IFO 3925 – – – – – – 0.53 0.11 – 0.62 * 0.14 * 0.09 * 27 Pseudomonas sp. strain K-9 TPU 7177 0.52 0.78 – 2.03 * 1.31 * 0.76 * 0.29 0.12 – 0.23 0.39 0.41 25 Rhodococcus erythropolis BG-13 TPU 3220 0.19 0.14 – 0.40 * 0.84 * 0.55 * 0.50 0.75 – 1.47 * 0.28 * 0.10 * Asano et al. Rhodococcus erythropolis BG-16 TPU 3221 1.71 1.15 – 1.58 * 1.53 * 0.31 * – – – – – – Asano et al. Rhodococcus rhodochrous J-1 TPU 3311 2.08 0.17 – 13.4 * 10.8 * 3.81 * 0.43 0.11 – 7.52 1.38 1.73 3 , 8 , 19 Rhodococcus rhodochrous NCIMB 11273 (ATCC 21197) – – – – – – 0.47 0.16 – 0.54 * 0.13 * 0.04 * 11 Rhodococcus sp. strain AK-32 TPU 3466 1.4 6.3 – 28.0 * 13.8 * 1.15 * 1.15 0.12 – 189 9.5 – 27 Rhodococcus sp. strain I-9 TPU 3467 0.72 0.16 – 1.25 0.61 1.33 0.84 0.12 – 0.31 14.6 3.75 24 Rhodococcus sp. strain N-774 TPU 3465 7.97 27.2 – 208 * 158 * 70.2 * 7.02 0.81 – 42.0 * 17.6 * 23.5 * 23 Rhodococcus sp. strain NCIMB 11215 – – – – – – 1.44 1.33 – 3.23 1.80 0.52 10 Rhodococcus sp. strain NCIMB 11216 – – – – – – 0.25 0.31 – 1.62 0.97 0.32 9 Fusarium merismoides TG-1 TPU 4500 0.36 0.17 – 2.07 4.26 3.86 0.39 0.25 – 0.11 0.10 0.12 2 Fusarium solani TG-2 TPU 4501 1.05 0.18 – 4.53 2.65 2.35 0.79 0.10 – 0.10 0.37 0.18 2 Candida guilliermondii IFO 0454 – – – – – – 0.11 0.10 – 0.85 * 0.10 * 0.14 * 28 Rhodococcus sp. strain YH3-3 c TPU 3453 22.4 4.21 – 288 * 89.3 * 1.17 * 2.83 0.08 – 144 * 56.3 * 0.15 * 7 Bacillus sp. strain OxB-1 d TPU 5563 12.2 0.91 – 2.59 4.11 0.94 – – – – – – 12 Open in a separate window a The strains were grown with PAOx, PAN, PyOx, or CyPy or without inducers.

Techniques: Activity Assay